primary antibodies against ki67 9027  (Cell Signaling Technology Inc)

Journal: Genes & Diseases

Article Title: NKAPL suppresses NSCLC progression by enhancing the protein stability of TRIM21 and further inhibiting the NF-κB signaling pathway

doi: 10.1016/j.gendis.2025.101598

Figure Lengend Snippet: NKAPL suppressed NSCLC growth and metastasis in vivo . (A) A549 cells stably expressing the vector and NKAPL-GFP were subcutaneously injected into BALB/c nude mice. Images of the subcutaneous tumors of the nude mice were captured. (B, C) The volume of the subcutaneous tumors in the nude mice was measured, and the tumors were weighed. (D) Hematoxylin-eosin staining and the expression changes of NKAPL and Ki-67 in xenograft tumors were examined by immunohistochemistry staining. (E, F) Representative bioluminescence images of the metastatic mouse models at the indicated times imaged by an in vivo imaging system. (G) Hematoxylin-eosin-stained sections showed pulmonary metastatic foci indicating A549-Luc cells in lung tissue sections. The data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: The sections were incubated with primary antibodies against GFP (sc-9996; Santa Cruz) and Ki67 (sc-23900, 1:200 dilution).

Techniques: In Vivo, Stable Transfection, Expressing, Plasmid Preparation, Injection, Staining, Immunohistochemistry, In Vivo Imaging, Standard Deviation

Journal: Drug Design, Development and Therapy

Article Title: Modified Huanjingjian Prevents Chemotherapy-Induced Alopecia by Inhibiting Genomic DNA Methylation of the Wnt Signaling Pathway in Mice

doi: 10.2147/DDDT.S523809

Figure Lengend Snippet: Effects of MHJJ on HFSCs and cell proliferative gene Ki67 in histological skin sections (magnification, 200×; scale bar, 50 µm). ( A ) MHJJ could repair damaged HFSCs in CTX-treated mice. ( B ) Ki67 expressed higher in the dorsal skin of mice after MHJJ treatment (n = 5). Significant differences between the CTX and MHJJ groups were denoted by * ( P < 0.05), ** ( P < 0.01), and *** ( P < 0.001). The yellow arrows indicated the positive staining of Ki67.

Article Snippet: The primary antibody against Ki67 ( GB121141 , Servicebio, Wuhan, China) was diluted in 5% BSA and applied to each section (approximately 100 μL) for overnight incubation at 4 °C.

Techniques: Staining